Journal: Applied Microbiology and Biotechnology

Article Title: Development and characterization of mouse monoclonal antibodies targeting to distinct epitopes of Zika virus envelope protein for specific detection of Zika virus

doi: 10.1007/s00253-021-11364-1

Figure Lengend Snippet: Alignment of the binding epitopes of mAbs A1 ( a ), B1 ( b ), C1 ( c ), and 9E-1 ( d ) with other flavivirus E proteins. DENV, dengue virus; WNV, West Nile virus; YFV, yellow fever virus; JEV, Japanese encephalitis virus. The NCBI accession numbers of the E proteins derived for the indicated virus strains are provided in the parentheses. e The recombinant E proteins of ZIKV (2 μg), DENV2 (1.75 μg), DENV3 (0.42 μg), and DENV4 (0.78 μg) were separated on SDS-PAGE and then transferred to a PVDF membrane for performing the WB analysis with mAbs Anti-His, A1, B1, C1, or 9E-1, respectively. The numbers labeled on the left of the designated ticks are the molecular weight standards in kDa unit

Article Snippet: The recombinant DENV serotype 2 (DENV2) E protein (GenBank: AAC59274.1), serotype 3 (DENV3) E protein (GenBank: AAA99437.1), and serotype 4 (DENV4) E protein (GenBank: AAX48017.1) were purchased from Sino Biological Inc. (Catalog Number: 40471-V08B, 40532-V08H1, and 40533-V08B2, respectively).

Techniques: Binding Assay, Virus, Derivative Assay, Recombinant, SDS Page, Membrane, Labeling, Molecular Weight